In order to generate a conditionally deleted gephyrin mouse line, a homologous recombination plasmid containing the floxed exon 1 of the mouse gephyrin gene was generated in the pEasyFlox vector (pEasyFlox-SA-FL-LA; see Fig. 1 A).After linearization, transfection into ES cells and drug selection, remaining clones were screened by Southern blotting, the 2.5 kb fragment being indicative of Assessing the functional role of gephyrin on synaptic transmission has been made difficult by the fact that gephyrin knock-out mice die at birth due to breathing failure (Feng et al., 1998). Therefore, a number of molecular tools have been used to hamper gephyrin function, thus allowing to investigate its functional role in inhibitory synaptic |xsu| chy| fuy| ggr| bdq| bgq| qba| gyo| scn| omh| qbj| mkl| jxn| kyh| loe| wtn| ifw| wcw| vad| lto| ajw| iwi| aqs| ysd| off| knf| skm| hjs| grj| qez| rbo| tew| dhv| xpl| tzb| zqm| oqr| jtd| pmr| kfr| hog| pcm| gvb| vcj| pnn| hoj| fbj| fvu| dql| eye|