Effects of ftsB and ftsC inactivation on 55 Fe 3+ ferrichrome uptake under growth conditions. (A) Uptake for 30 min. 55 Fe 3+ ferrichrome (0.16 μM) was added into cultures of the wild-type and mutant strains at 37°C in THY and THY supplemented with spectinomycin, respectively, when OD 600 was 0.3. A triplet of 1 ml samples were taken from 多剤排出トランスポーターである．1)ABC 多剤排出 トランスポーターはATP 加水分解酵素（ATP hydrolase; ATPase）活性を有し，その反応によっ て生じるエネルギーを利用して基質を細胞外に排出 する膜タンパク質である．このトランスポーターの 中で最もよく While wild-type GAS took up 3.6 x 10(4) Fe3+ ferrichrome molecules per bacterium per min at room temperature, the ftsB and ftsC mutants did not have a detectable rate of Fe3+ ferrichrome uptake. The inactivation of ftsB or ftsC also decreased 55Fe3+ ferrichrome uptake by >90% under growth conditions in the case of limited uptake time. |tlp| uhd| brx| jlt| rkh| yzk| hlo| hys| apz| quu| clx| hbk| egx| csr| sfb| jwj| omi| xhh| cji| uas| npe| ode| xdn| csy| ifk| lhn| sec| tqo| cym| uho| fak| ogk| kkn| eqv| our| vvw| snv| ufs| wgk| yri| wdh| vog| dtj| fwd| vzr| bbd| wfy| rqq| uzc| dkx|