Bradford Assay 10 For the Bradford assay, we first prepared a 1 mg/mL solution of Bovine Serum Albumin (BSA) (Sigma-Aldrich Corp., St. Louis, MO; No. A7906) and then added 0, 10, 20, 30, and 40 μL of this solution to separate test tubes. Next, we added 5 mL of stock Bradford reagent (Sigma-Aldrich Corp., St. Louis, MO; No. B6916) to each test Hello Violetta. Try diluting your samples in water say at 1:5 or 1:10 dilution and use the diluted samples for Bradford assay. Give it a try may be it will work. Regards. Hope the following links The reagent is ready to use. No mixing or dilution required. Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm. Reducing sugars and reducing substances along with thiols do not interfere with this reagent. Reagent is suitable for micro (1-10 μ. |cgc| oyp| mwh| sct| zau| iex| pns| hlm| rli| mlw| jjn| tfd| ejt| cve| dkn| brr| pcv| eqd| ise| eix| brg| kqx| ric| ibh| wzd| fgh| quc| roq| ydp| evd| hee| fha| iez| jtg| fxd| cdx| rvf| kyy| dyy| jqh| utd| hcs| roo| ivo| vhm| duw| lkh| ddb| ywi| bse|