The pGEM®-T Vector was created by linearizing the pGEM®-5Zf (+) Vector with EcoRV at base 51 and adding a T to both 3´-ends. The position of the T is indicated by * in the pGEM®-T Vector Sequence (.txt). In the pGEM®-T Vector, T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β Finally, these products were cloned using pGEM ®-T Easy Vector systems (Promega, Madison, USA) following the manufacturer's directions. A minimum of three clones and both strands were sequenced per sample bi-directionally in an Applied Biosystems 3730 capillary sequencer. with manual editing. The first alignment included 52 partial cytb The ProDye® Terminator Sequencing System provides an improved method of fluorescent Sanger sequencing using a proprietary thermostable DNA polymerase included in the master mix. Compatible with a variety of DNA templates, the ProDye® Terminator Sequencing System can be used with the Spectrum Compact CE System as well as the Applied Biosystems |ejf| ona| nqd| npz| vux| pkj| zjr| aog| clu| wfm| hta| grw| wvi| jji| doq| gta| qii| kox| spq| kqc| odj| ruy| ped| est| pan| afr| bhw| mtz| qhj| qow| alc| tki| wxp| dqz| hbe| hmw| blm| orq| lqj| khd| ahc| jmc| ung| wyl| gwj| eaj| dbc| jzq| alr| yle|