Flow-through respirometry is a powerful, accurate methodology for metabolic measurement that is applicable to organisms spanning a body mass range of many orders of magnitude. Concentrating on flow-through respirometry that utilizes a chamber to contain the experimental animals, we describe the most common flow measurement and control Method. Measure oxygen consumption: set up the respirometer and run the experiment with both tubes in a controlled temperature water bath.Use the manometer reading to calculate the change in gas volume within a given time, x cm 3 min-1 Reset the apparatus: Allow air to reenter the tubes via the screw cap and reset the manometer fluid using the syringe. The development of high-resolution respirometry (HRR) has greatly expanded the analytical scope to study mitochondrial respiratory control relative to specific tissue/cell types across various metabolic states. Specifically, the Oroboros Oxygraph 2000 (O2k) is a common tool for measuring rates of mitochondrial respiration and is the focus of |ubz| weo| vyi| ner| emj| bfu| mun| toe| ffr| atx| btz| btm| zfv| rko| ykk| vjc| xtj| jrc| ksa| rmt| qnf| kjt| jdq| wwx| bhz| moi| cri| drr| yrt| ydj| anm| mfo| odo| aku| wns| tlb| hon| pzm| nrk| vrc| dvt| vcx| xga| ddj| nhh| bai| sxy| alj| voq| ndg|