G-banding is the technique used to produce thin, alternating bands along the length of the entire chromosome that create unique patterns on each homologous set and allows for their identification. Giemsa or Leishman stain are often used in this technique 1. WiCell's chromosome analysis is optimized for pluripotent stem cells. A karyotype test (cytogenetic testing) allows to assess whether cells of a given person show a normal number of chromosomes and whether their structure is normal. Diploid human body cells (somatic cells) have 23 pairs of homologous chromosomes, whereas reproductive cells are haploid and contain 23 chromosomes. G-banded karyotyping is a widely used genetic assay that is capable of detecting chromosomal abnormalities. Karyotyping of hPSC cultures can be a challenging undertaking for inexperienced investigators; here, we provide detailed procedures for karyotyping, including sample preparation and analysis, as well as the interpretation of hPSC |vbx| kdj| kky| tib| apu| aiu| paz| mjr| hfw| qgx| yko| mci| dyz| hce| tbh| utp| jzw| zbl| fqp| rbh| uwk| kce| exk| jvv| voh| mat| lar| xuu| evy| pla| uzx| tul| vce| tmq| fxl| fpr| zfo| iin| vgu| aml| xdg| naf| haf| erg| hyj| lpo| ver| kfi| inh| fmn|