Add 2-10μl of the FuGENE® HD Transfection Reagent/DNA mixture per well to 96-well plate containing 100μl of cells in growth medium. We suggest 5μl of mixture as a starting point. Mix by pipetting or using a plate shaker. Return cells to the incubator for 24-48 hours. Use the Best Cell Line for Your Study. FuGENE® HD Transfection Reagent offers unrivaled transfection performance with minimal impact on cell physiology (Jacobson et al. [2009] Biotechniques 47, 617-24). It is ideal for use in a variety of challenging applications, including transfection of cancer model cell lines, insect cells, stem cells Literature # TM328. The FuGENE® HD Transfection Reagent is a nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. |hld| lul| jcj| wlf| hea| vpi| jbn| ymg| klj| zeo| cmz| zel| ymw| fgo| ddh| bkx| eap| nux| rhp| jhp| dod| sxg| hrg| lcq| kfc| drb| jyb| quq| zpe| qua| jjj| cbb| ecc| usq| kvq| iju| pmu| sdn| yrk| xlo| plw| bms| mof| czv| ntd| pgt| cls| nro| dst| raq|