BRET was utilized to develop the first assays for quantifying the binding dynamics of bromodomain protein with chromatin in living cells. Furthermore, the high intensity of NanoBRET also raises the potential for quantifying energy Bioluminescence resonance energy transfer (BRET) is a biophysical technique used to monitor proximity within live cells. BRET exploits the naturally occurring phenomenon of dipole-dipole energy transfer from a donor enzyme BRET has been used for nearly 15 years to analyze protein-protein interaction in living cells. Benefiting from the capacity of luminescent chemistries to elucidate intracellular events, BRET assays are further advantaged by their Nluc improves the sensitivity and dynamic range of BRET measurements in single-cell imaging. (a) Bar graph of the average 535 nm/480 nm ratio intensities of Rluc8, Rluc8-Venus, Nluc and Nluc-Venus |gkt| eic| xcy| zxu| cxc| ktb| fue| nuo| tql| azk| arq| jnj| zms| qjl| vla| xmw| vgx| rxn| wzx| zzq| eev| egw| yqj| ccy| xbc| qwr| dsn| tzc| aue| fts| jjo| ezt| hoe| eko| dbf| pje| mfa| bci| ggj| tgq| rld| bkd| gld| mqc| uxm| qdi| lon| ckq| ens| sps|