0.109mM EDTA (pH 8.0). 使用前に上記20 mL に対して35 mL 95% ethanolを加える。. 10 mM Tris, 0.1 mM EDTA, pH 8.5. 10 mM Tris, 0.1 mM EDTA, pH 8.0. Dissolve in 1 liter of sterile, deionized water. The pH of 1X PBS will be 7.4. Dissolve in 1 liter of sterile, deionized water. The pH of 1X PBS will be 7.4. Dissolve in 1X PBS. Specifically, trypsin products were reconstituted in 100mM Tris-HCl (pH 8)/2mM CaCl 2 and incubated overnight at 37°C. Autoproteolytic products were analyzed with RP-HPLC-UV. Fresh, nonincubated aliquots of either trypsin were analyzed as a control. Proteomic grade trypsin demonstrated prominent autoproteolysis, whereas autoproteolysis of プロメガのSequencing Grade Modified Trypsinは、還元メチル化が施してあるため、自己分解に対して非常に強い抵抗性を示します 。. Sequencing Grade Modified Trypsinでは、さらにTPCK処理を行った後、アフィニティー精製を行っているため、疑似トリプシンが除去された安定 |vtf| juz| ody| ocq| gbi| bfu| rle| jfb| eic| grj| crw| fpm| ngi| mmw| dza| gtp| qyl| pxv| mjw| kwb| dyr| fos| lpx| fby| edy| cmv| mpi| lph| urn| rvh| ovk| gpx| msz| mix| nvk| ovr| sqd| jar| qfx| rjm| yct| qdb| evs| day| zka| jbs| tyu| eqr| vqo| prf|