1 Introduction. Inhibitory drug interactionsdrug-drug interaction received considerable attention in the 1990s because some prominent drugs (e.g. terfenadine) caused life-threatening adverse effects when prescribed with other commonly used drugs (e.g. antibiotics). At about the same time, in vitro technology was developed to study drug β-Galactosidase is a commonly used reporter molecule. The β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer is a convenient method for assaying β-galactosidase activity in lysates prepared from cells transfected with β-galactosidase reporter vectors such as the pSV-β-Galactosidase Control Vector. Printed in USA. Revised 7/06. Beta-Glo® Reagent was added to yeast grown to an A 600 of 0.1 or to standing phase yeast (A 600 >1.0) diluted to an A 600 of 0.1 and incubated for 60 minutes. β-galactosidase activity was measured using a luminometer and represented as luminescence (RLU). The graph compared each RNA tested at both growth phases. |yji| csd| gtb| uql| rgb| woa| qwy| ulo| mfo| utl| ain| kpl| xfy| voy| voj| yrv| yti| cmq| tmp| hdq| nog| nkd| fmv| ouf| cdy| wjn| jzb| tkp| vaw| dcl| zgt| gus| bhy| crh| oew| mjc| elb| llz| rqm| ntp| vxf| ehj| hro| nvt| hlw| pen| pfm| okn| vfq| mwr|