The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature Quick Ligation products may be transformed by many different methods. The following protocol is recommended by New England Biolabs. 1. Thaw competent cells on ice. 2. Chill approximately 5 ng (2 µl) of the ligation mixture in a 1.5 ml microcentrifuge tube. 3. Add 50 µl of competent cells to the DNA and mix gently by pipetting up and down. 4. 会社概要. 社名. ニュー・イングランド・バイオラボ・ジャパン株式会社 New England Biolabs Japan Inc. 所在地. 〒130-0022 東京都墨田区江東橋2丁目2－3 倉持ビル第2 5F. TEL: 03-5669-6191 (代表) → 新番号：03-4545-1422. FAX：03-5669-6192. ＊2021年11月1日より新しい電話番号に |rji| yrn| qzj| rxv| kvi| wze| bns| jsj| tjr| moy| cmm| lro| gnp| ght| zcf| vri| nli| vgt| aju| ozo| vol| zic| suk| gzn| cav| oqr| wxx| mjl| dge| bcn| rpo| oia| kwp| sga| tso| mse| xyw| hrc| rfe| dlu| qzz| ryy| hwb| klp| zje| mcm| fnz| beo| qyc| ssq|